A novel, nitric oxide-releasing elastomeric chain for antimicrobial action: proof of concept

Introduction. Microbial colonization of orthodontic elastomeric chains is a two-fold problem: (1) plaque promotes carious lesions during orthodontic treatment and (2) bacterial by-products can degrade a chain's mechanical properties. Efforts to combat this colonization have included the development of materials with antimicrobial activity. Recently, biomedical research has focused on the antibacterial properties of S-Nitroso-N-acetylpenicillamine (SNAP), a synthetic Nitric Oxide (NO) donor that exhibits extended NO release when incorporated into low water-uptake polymers. The objective of this study is to generate an antibacterial orthodontic elastomeric chain using this technology. Methods. Elastomeric power chain is impregnated with the SNAP molecule and chains are evaluated for their Nitric Oxide release kinetics. The chains are then tested for their antibacterial efficacy against a common oral pathogen, Streptococcus mutans. Results. Existing elastomeric chains are successfully impregnated with SNAP and show NO-release over a three-day period. Experimental chains demonstrate good S. mutans inhibition on and around the chain surface over a 48 h period. Conclusions. Nitric Oxide-Release technology can be applied to products in the dental field and may allow for biomimetic materials that would help to reduce bacteria-related pathologies such as white spot lesions and gingivitis.