Study on the extraction and back extraction of Bovine Serum Albumin using reversed micelles

被引：5
|
作者：
Li, Xiangcun [1 ]
He, Gaohong [1 ]
Lin, Chang [1 ]
Liu, Hongjing [1 ]
机构：
[1] Dalian Univ Technol, R&D Ctr Membrane Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2007年 / 42卷 / 16期
基金：
中国国家自然科学基金;
关键词：
BSA; revered micelles; extraction ratio; selectivity;
D O I：
10.1080/01496390701626750
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The extraction and back extraction of Bovine Serum Albumin (BSA) using a new reversed micelle system of CTAB/isooctane/1-pentanol have been investigated. Under the optimal operating conditions in this study, the extraction ratio of BSA reaches as high as 98%. The back extraction ratio can reach 80% as pH in stripping phase approaches to the isoelectric point (pI) of BSA. UV-spectra indicated that there was no significant change in the structure of BSA enriched in stripping phase. Furthermore, the reversed micelle system was repeatedly used to extract BSA from a mixed solution of protein.
引用
收藏
页码：3741 / 3757
页数：17
相关论文
共 50 条
  • [41] Reverse Micelle Liquid-Liquid Extraction of Bovine Serum Albumin and Lysozyme
    Mohd-Setapar, Siti Hamidah
    Mohamad-Aziz, Siti Norazimah
    Joanne, Constantine
    JURNAL TEKNOLOGI, 2012, 58
  • [42] Application of Aqueous Two-phase Systems in the Extraction of Bovine Serum Albumin
    Mahdi, Hawraa A.
    Hameed, Khalid W.
    Ali, Abdul-Jabbar A.
    JOURNAL OF POLYMER & COMPOSITES, 2023, 11 (01) : 41 - 50
  • [43] Effect of the spacer of gemini surfactants on reverse micellar extraction of bovine serum albumin
    Dong, Jin
    Cai, Juan
    Guo, Xia
    Xiao, Jing
    SOFT MATTER, 2013, 9 (47) : 11383 - 11391
  • [44] Extraction and re-extraction of phenylalanine by cationic reversed micelles in hollow fibre contactors
    Departamento de Química, Faculdade Cie. Tecn. Da Univ. N., 2825 Monte de Caparica, Portugal
    J. Membr. Sci., 2 (303-319):
  • [45] Extraction and re-extraction of phenylalanine by cationic reversed micelles in hollow fibre contactors
    Cardoso, MM
    Viegas, RMC
    Crespo, JPSG
    JOURNAL OF MEMBRANE SCIENCE, 1999, 156 (02) : 303 - 319
  • [46] EXTRACTION OF HYPEROSIDE FROM Hypericum perforatum L. USING CTAB REVERSED MICELLES
    Dong, Lichun
    Zhou, Xiaohua
    Li, Duanhua
    CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 2009, 87 (04): : 584 - 590
  • [47] MASS-TRANSFER RATE OF PROTEIN EXTRACTION WITH REVERSED MICELLES
    DEKKER, M
    VANTRIET, K
    BIJSTERBOSCH, BH
    FIJNEMAN, P
    HILHORST, R
    CHEMICAL ENGINEERING SCIENCE, 1990, 45 (09) : 2949 - 2957
  • [48] Liquid-liquid extraction by reversed micelles in biotechnological processes
    Kilikian, BV
    Bastazin, MR
    Minami, NM
    Gonçalves, EMR
    Pessoa, A
    BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2000, 17 (01) : 29 - 38
  • [49] Recent Developments of Reverse Micellar Techniques for Lysozyme, Bovine Serum Albumin, and Bromelain Extraction
    Chia, Shir Reen
    Tang, Malcolm S. Y.
    Chow, Yin Hui
    Ooi, Chien Wei
    Rambabu, Krishnamoorthy
    Zhu, Liandong
    Show, Pau Loke
    MOLECULAR BIOTECHNOLOGY, 2019, 61 (10) : 715 - 724
  • [50] Recent Developments of Reverse Micellar Techniques for Lysozyme, Bovine Serum Albumin, and Bromelain Extraction
    Shir Reen Chia
    Malcolm S. Y. Tang
    Yin Hui Chow
    Chien Wei Ooi
    Krishnamoorthy Rambabu
    Liandong Zhu
    Pau Loke Show
    Molecular Biotechnology, 2019, 61 : 715 - 724
12345