Study on the extraction and back extraction of Bovine Serum Albumin using reversed micelles

被引：5
|
作者：
Li, Xiangcun [1 ]
He, Gaohong [1 ]
Lin, Chang [1 ]
Liu, Hongjing [1 ]
机构：
[1] Dalian Univ Technol, R&D Ctr Membrane Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2007年 / 42卷 / 16期
基金：
中国国家自然科学基金;
关键词：
BSA; revered micelles; extraction ratio; selectivity;
D O I：
10.1080/01496390701626750
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The extraction and back extraction of Bovine Serum Albumin (BSA) using a new reversed micelle system of CTAB/isooctane/1-pentanol have been investigated. Under the optimal operating conditions in this study, the extraction ratio of BSA reaches as high as 98%. The back extraction ratio can reach 80% as pH in stripping phase approaches to the isoelectric point (pI) of BSA. UV-spectra indicated that there was no significant change in the structure of BSA enriched in stripping phase. Furthermore, the reversed micelle system was repeatedly used to extract BSA from a mixed solution of protein.
引用
收藏
页码：3741 / 3757
页数：17
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