A sensitive LC-MS assay using derivatization with boron trifluoride to quantify curcuminoids in biological samples

被引：8
|
作者：
Yoon, Alexander J. [1 ,2 ]
Wu, Haiqiang [1 ,2 ,3 ]
Pan, Roy D. [4 ,5 ]
Teter, Bruce [6 ]
Cipolla, Jack [1 ,2 ]
Chang, Edwin [7 ]
Avila, Luis Z. [8 ]
Basak, Saroj K. [9 ,10 ,11 ]
Srivatsan, Eri S. [9 ,10 ,11 ,12 ]
Wang, Marilene B. [12 ,13 ]
Cole, Greg M. [6 ]
Frautschy, Sally A. [6 ]
Hampton, Phillip D. [13 ]
Faull, Kym F. [1 ,2 ]
机构：
[1] Univ Calif Los Angeles, David Geffen Sch Med, Jane & Terry Semel Inst Neurosci & Human Behav, Pasarow Mass Spectrometry Lab, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90095 USA
[3] Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Shenzhen 518060, Peoples R China
[4] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
[5] Univ Calif Los Angeles, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA
[6] Univ Calif Los Angeles, David Geffen Sch Med, Dept Neurol, Los Angeles, CA 90095 USA
[7] Stanford Univ, Dept Radiol, Stanford, CA 94305 USA
[8] Aveta Biom, Bedford, MA 01730 USA
[9] Vet Adm Greater Angeles Hlth Syst, Dept Surg, Los Angeles, CA 90073 USA
[10] Univ Calif Los Angeles, David Geffen Sch Med, Dept Surg, Los Angeles, CA 90073 USA
[11] Vet Adm Greater Angeles Hlth Syst, Dept Surg, Los Angeles, CA 90073 USA
[12] Univ Calif Los Angeles, David Geffen Sch Med, Dept Head & Neck Surg, Los Angeles, CA 90073 USA
[13] Calif State Univ Channel Isl, Dept Chem, Camarillo, CA 93012 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2020年 / 596卷
关键词：
Curcumin; Curcuminoids; Liquid chromatography coupled mass spectrometry (LCMS); Pharmacokinetics; Boron trifluoride (BF3); RP-HPLC METHOD; RAT PLASMA; BISDEMETHOXYCURCUMIN; DEMETHOXYCURCUMIN; QUANTIFICATION; ACID;
D O I：
10.1016/j.ab.2020.113636
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves converting the analyzes to their boron difluoride derivatives and analyzing them by combined liquid chromatography coupled to an ion trap mass spectrometer operating in the negative ion MSn scan mode. The method has superb limits of detection of 0.01 nM for all curcuminoids and 0.5 nM for TC and the glucuroniated metabolites, and several representative chromatograms of biological samples containing these analyzes are provided. In addition, the pharmacokinetic profile of these compounds in one human who daily consumed an over-the-counter curcuminoid product shows the peak and changes in circulating concentrations achieved by this mode of administration.
引用
收藏
页数：9
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