Bone Regeneration Using Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

被引:0
|
作者
Lee, Sang Uk [1 ]
Chung, Yang Guk [1 ]
Oh, Il Hoan [2 ]
Kim, Jung Man [1 ]
Kim, Yang Soo [1 ]
Lee, Yun Gyoung [1 ]
Baek, Myong Hyun [3 ]
Kim, Sung Fun [1 ]
机构
[1] Catholic Univ Korea, Coll Med, Dept Orthoped Surg, Seoul 137701, South Korea
[2] Catholic Univ Korea, Coll Med, Catholic High Performance Cell Therapy Ctr, Seoul 137701, South Korea
[3] BK Meditech Co Ltd, Kyonggi Do 445930, South Korea
关键词
bone regeneration; allogeneic cancellous bone granule; mesenchymal stem cells; micro-CT; CRITICAL-SIZE DEFECTS; STROMAL CELLS; DIFFERENTIATION; MEDICINE;
D O I
暂无
中图分类号
Q813 [细胞工程];
学科分类号
摘要
For developing an effective bone regeneration strategy, we studied the efficacy of bone regeneration using allogeneic cancellous bone granules loaded with bone marrow-derived autologous MSCs in NWR radius. In the experimental group, allogeneic cancellous bone granules and co-cultured MSCs were implanted in one forearm. Allogeneic cancellous bone granules were grafted into the other side in control group. For quantitative assessment of bone regeneration, radiographic evaluation, and BMD/BMC measurements were performed at 3, 6, 9 and 12 weeks, and histology, micro-CT image analysis were performed at 6 and 12 weeks post-implantation. On radiographic evaluation, the experimental group showed higher bone quantity index at 6 and 9 weeks post-implantation (p<0.05), but the difference was decreased at 12 weeks. On micro-CT image analysis, the experimental group revealed more total volume and surface area of regenerated bone than the control group. The bone volume at 6 weeks reached statistical significance. Histological evaluation of 6 week-specimens revealed much more resorption of grafted granules and new bone formation in the experimental group. In conclusion, the present in-vivo study demonstrated that combining co-cultured autologous MSCs with small-sized allogeneic cancellous bone granules resulted in more and accelerated bone regeneration, especially in the early phase.
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页码:401 / 409
页数:9
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