The central PPT of the yeast retrotransposon Ty1 is not essential for transposition

被引：11
|
作者：
Heyman, T [1 ]
Wilhelm, M [1 ]
Wilhelm, FX [1 ]
机构：
[1] CNRS, Inst Biol Mol & Cellulaire, UPR9002, F-67084 Strasbourg, France
来源：
JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 331卷 / 02期
关键词：
Ty1; retrotransposon; polypurine tract; transposition; yeast;
D O I：
10.1016/S0022-2836(03)00812-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The yeast retrotransposon Ty1 has structural and functional similarities to retroviruses. We report here that, as in retroviruses, the plus-strand DNA of Ty1 is synthesized as two segments. A central DNA flap is formed during reverse transcription consecutive to elongation (with strand displacement) of the upstream segment beyond the central polypurine tract (cPPT) until the replication machinery is stopped at the central termination sequence. Comparison of wild-type and cPPT-mutant Ty1 elements shows that the mutant element lacking the central DNA flap is only twofold defective in transposition. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：315 / 320
页数：6
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