Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

被引：10
|
作者：
Woods, Jared K. [1 ]
Kowalski, Suzanne [1 ]
Rogina, Blanka [1 ]
机构：
[1] Univ Connecticut, Ctr Hlth, Sch Med, Storrs, CT 06269 USA
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2014年 / 86期
基金：
美国国家卫生研究院;
关键词：
Neuroscience; Issue; 86; Investigative Techniques; Life Sciences (General); Behavioral Sciences; Drosophila melanogaster; Fruit flies; Spontaneous physical activity; Mobility; Fly behavior; Locomotor Activity; AGE-RELATED IMPAIRMENT; NEGATIVE GEOTAXIS; SYSTEM; FLY;
D O I：
10.3791/51449
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Drosophila melanogaster has been used as an excellent model organism to study environmental and genetic manipulations that affect behavior. One such behavior is spontaneous locomotor activity. Here we describe our protocol that utilizes Drosophila population monitors and a tracking system that allows continuous monitoring of the spontaneous locomotor activity of flies for several days at a time. This method is simple, reliable, and objective and can be used to examine the effects of aging, sex, changes in caloric content of food, addition of drugs, or genetic manipulations that mimic human diseases.
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页数：6
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