In Vitro Selection of Diversely Functionalized Aptamers

被引：64
|
作者：
Kong, Dehui [1 ]
Yeung, Wayland [1 ]
Hili, Ryan [1 ,2 ]
机构：
[1] Univ Georgia, Dept Chem, 140 Cedar St, Athens, GA 30602 USA
[2] York Univ, Dept Chem, 4700 Keele St, Toronto, ON M3J 1P3, Canada
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2017年 / 139卷 / 40期
基金：
美国国家卫生研究院;
关键词：
NUCLEIC-ACID POLYMERS; MODIFIED DNA; ENZYMATIC-SYNTHESIS; HUMAN THROMBIN; LIGANDS; BIND; ENRICHMENT; MOLECULES; FIDELITY; CHAIN;
D O I：
10.1021/jacs.7b07241
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We describe the application of T4 DNA ligase-catalyzed DNA templated oligonucleotide polymerization toward the evolution of a diversely functionalized nucleic acid aptamer for human a-thrombin. Using a 256-membered ANNNN comonomer library comprising 16 sublibraries modified with different functional groups, a highly functionalized aptamer for thrombin was raised with a dissociation constant of 1.6 nM. The aptamer was found to be selective for thrombin and required the modifications for binding affinity. This study demonstrates the most differentially functionalized nucleic acid aptamer discovered by in vitro selection and should enable the future exploration of functional group dependence during the evolution of nucleic acid polymer activity.
引用
收藏
页码：13977 / 13980
页数：4
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