Integrated Microfluidic Sample-to-Answer System for Direct Nucleic Acid-Based Detection of Group B Streptococci in Clinical Vaginal/Anal Swab Samples

被引:23
|
作者
Ye, Xin [1 ,2 ]
Li, Yang [3 ,4 ]
Fang, Xueen [1 ,2 ]
Kong, Jilie [1 ,2 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
[3] Shanghai Suxin Biotechnol Co Ltd, Shanghai 201318, Peoples R China
[4] Suchuang Diagnost Prod Co Ltd, Shanghai 201318, Peoples R China
来源
ACS SENSORS | 2020年 / 5卷 / 04期
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
group B Streptococcus; nucleic acid-based detection; sample-to-answer detection; microfluidic; isothermal amplification; INTRAPARTUM ANTIBIOTIC-PROPHYLAXIS; ISOTHERMAL AMPLIFICATION; UNITED-STATES; POINT; GENE; COLONIZATION; DISEASE;
D O I
10.1021/acssensors.0c00087
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The group B Streptococcus (GBS) is a type of pathogen that seriously threatens the health of mothers and infants. Prompt and timely diagnosis is crucial for good patient outcomes. However, the traditional bacterial culture and polymerase chain reaction methods are limited by their speed and involve complex operating procedures. Herein, we successfully established an integrated microfluidic sample-to-answer system for nucleic acid-based detection of GBS directly in vaginal/anal swab samples. Meanwhile, we demonstrated a dynamical reaction mechanism of Bst/FEN1-based nucleic acid amplification, which differs from traditional Bst-based isothermal amplification strategies. The system integrates cell lysis and nucleic acid purification, separation, amplification, and detection, enabling rapid (about 45 min to the entire analysis) and highly accurate (98% accuracy) analysis in a clinical setting. Experimental results show that the system offers a good detection limit (500 CFU/mL), perfect specificity (no cross-reactivity with 25 other common pathogens), excellent stability (coefficient of variation less than 3%), and good anti-interference performance. This novel system holds great potential as a nucleic acid-based diagnostic tool in clinical applications for detecting not only GBS but also other types of pathogens.
引用
收藏
页码:1132 / 1139
页数:8
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