Detection of bioactive compounds from Spilanthes acmella (L.) plants and its various in vitro culture products

In this study, we analyzed the active chemical compounds of in vitro plantlets of Spilanthes acmella. With 19 samples, GC-MS (Gas Chromatography-Mass Spectrometry) analysis revealed that the naturally occurring insecticide, N-isobutyl-2E, 6Z, 8E-decatrienamide (spilanthol) was present in mother plant, flower heads and in vitro plantlets of S. acmella with similar retention time (43.18 to 43.21 min). N-isobutyl-2E, 4Z, 8Z, 10E-dodecatetraenamide (an isomer of N-isobutyl-2E, 4E, 8E, 10E-dodecatetraenamide, a potent mosquito larvicide) was only detected in the in vitro plantlets of S. acmella. Antioxidant, butylated hydroxytoluene (BHT) and fatty acids (n-Hexadecanoic acid and tetradecanoic acid) could be obtained from all the sample extracts of mother plant, flower heads, in vitro plantlets, callus, air-dried cells, freeze-dried cells and fresh cells.