Production of capsular polysaccharide of Streptococcus pneumoniae type 14 and its purification by affinity chromatography

被引：22
|
作者：
Suárez, N
Fraguas, LF
Texeira, E
Massaldi, H
Batista-Viera, F
Ferreira, F
机构：
[1] Inst Higiene, Fac Quim, Lab Carbohidratos & Glicocunjugados, Dept Quim Organ, Montevideo, Uruguay
[2] Inst Higiene, Dept Desarrollo & Prod, Fac Med, Montevideo, Uruguay
[3] Fac Quim, Catedra Bioquim, Montevideo, Uruguay
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 02期
关键词：
D O I：
10.1128/AEM.67.2.969-971.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.
引用
收藏
页码：969 / 971
页数：3
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