Start codon in the Bacillus intermedius gene for serine proteinase

被引：0
|
作者：
Kayuynov, A. R. [1 ]
Sabirova, A. R. [1 ]
Balaban, N. P. [1 ]
Mardanova, A. M. [1 ]
Ilinskaya, O. N. [1 ]
Kostrov, S. V. [2 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan VI Lenin State Univ, Fac Biol & Soil, Kazan 420008, Russia
[2] Russian Acad Sci, Inst Mol Genet, Moscow 119991, Russia
来源：
MOLECULAR BIOLOGY | 2008年 / 42卷 / 01期
关键词：
subtilisin-like serine protease; start codon; mutant genes; Bacillus intermedius;
D O I：
10.1134/S0026893308010159
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The translation initiation site was determined for Bacillus intermedius aprBi (AN AY754946), coding for extracellular subtilisin-like serine proteinase secreted at the stationary growth phase. Analysis of the aprBi open reading frame revealed three potential translation start sites (TTG, GTG, and ATG). Using the SignalP online freeware program, the probabilities of their functional activity were evaluated. To identify the translation start, modified subtilisin-like protease genes with nucleotide replacements in putative start codons were obtained by oligonucleotide-directed mutagenesis. The expression of these genetic constructs was investigated in protease-deficient strain B. subtilis AJ73. The results indicated that aprBi translation starts from the alternative GTG codon.
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收藏
页码：105 / 109
页数：5
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