Start codon in the Bacillus intermedius gene for serine proteinase

被引：0
|
作者：
Kayuynov, A. R. [1 ]
Sabirova, A. R. [1 ]
Balaban, N. P. [1 ]
Mardanova, A. M. [1 ]
Ilinskaya, O. N. [1 ]
Kostrov, S. V. [2 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan VI Lenin State Univ, Fac Biol & Soil, Kazan 420008, Russia
[2] Russian Acad Sci, Inst Mol Genet, Moscow 119991, Russia
来源：
MOLECULAR BIOLOGY | 2008年 / 42卷 / 01期
关键词：
subtilisin-like serine protease; start codon; mutant genes; Bacillus intermedius;
D O I：
10.1134/S0026893308010159
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The translation initiation site was determined for Bacillus intermedius aprBi (AN AY754946), coding for extracellular subtilisin-like serine proteinase secreted at the stationary growth phase. Analysis of the aprBi open reading frame revealed three potential translation start sites (TTG, GTG, and ATG). Using the SignalP online freeware program, the probabilities of their functional activity were evaluated. To identify the translation start, modified subtilisin-like protease genes with nucleotide replacements in putative start codons were obtained by oligonucleotide-directed mutagenesis. The expression of these genetic constructs was investigated in protease-deficient strain B. subtilis AJ73. The results indicated that aprBi translation starts from the alternative GTG codon.
引用
收藏
页码：105 / 109
页数：5
相关论文
共 50 条
  • [1] Start codon in the Bacillus intermedius gene for serine proteinase
    A. R. Kayumov
    A. R. Sabirova
    N. P. Balaban
    A. M. Mardanova
    O. N. Ilinskaya
    S. V. Kostrov
    M. R. Sharipova
    Molecular Biology, 2008, 42 : 105 - 109
  • [2] The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis
    Sharipova, Margarita
    Balaban, Nelly
    Kayumov, Airat
    Kirillova, Yuliya
    Mardanova, Ayslu
    Gabdrakhmanova, Leila
    Leshchinskaya, Inna
    Rudenskaya, Galina
    Akimkina, Tatiana
    Safina, Dina
    Demidyuk, Ilya
    Kostrov, Sergey
    MICROBIOLOGICAL RESEARCH, 2008, 163 (01) : 39 - 50
  • [3] Biosynthesis of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain
    Kirillova, YM
    Mikhailova, EO
    Balaban, NP
    Mardanova, AM
    Kayumov, AR
    Rudenskaya, GN
    Kostrov, SV
    Sharipova, MR
    MICROBIOLOGY, 2006, 75 (02) : 142 - 147
  • [4] Biosynthesis of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain
    Yu. M. Kirillova
    E. O. Mikhailova
    N. P. Balaban
    A. M. Mardanova
    A. R. Kayumov
    G. N. Rudenskaya
    S. V. Kostrov
    M. R. Sharipova
    Microbiology, 2006, 75 : 142 - 147
  • [5] Purification and characterization of serine proteinase 2 from Bacillus intermedius 3-19
    Balaban, NP
    Mardanova, AM
    Sharipova, MR
    Gabdrakhmanova, LA
    Sokolova, EA
    Rudenskaya, GN
    Leshchinskaya, IB
    BIOCHEMISTRY-MOSCOW, 2004, 69 (04) : 420 - 426
  • [6] Purification and Characterization of Serine Proteinase 2 from Bacillus intermedius 3-19
    N. P. Balaban
    A. M. Mardanova
    M. R. Sharipova
    L. A. Gabdrakhmanova
    E. A. Sokolova
    G. N. Rudenskaya
    I. B. Leshchinskaya
    Biochemistry (Moscow), 2004, 69 : 420 - 426
  • [7] Growth conditions and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain
    Yu. M. Kirillova
    E. O. Mikhailova
    N. P. Balaban
    A. M. Mardanova
    G. N. Rudenskaya
    S. V. Kostrov
    M. R. Sharipova
    Microbiology, 2006, 75 : 136 - 141
  • [8] Growth conditions and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain
    Kirillova, YM
    Mikhailova, EO
    Balaban, NP
    Mardanova, AM
    Rudenskaya, GN
    Kostrov, SV
    Sharipova, MR
    MICROBIOLOGY, 2006, 75 (02) : 136 - 141
  • [9] Enzymatic properties of thiol-dependent serine proteinase of Bacillus intermedius 3-19
    Itskovich, EL
    Balaban, NP
    Mardanova, AM
    Shakirov, EV
    Sharipova, MR
    Leshchinskaya, IB
    Ksenofontov, AL
    Rudenskaya, GN
    BIOCHEMISTRY-MOSCOW, 1997, 62 (01) : 49 - 53
  • [10] Biosynthesis of the subtilisin-like serine proteinase of Bacillus intermedius under salt stress conditions
    Kayumov, A. R.
    Balaban, N. P.
    Mardanova, A. M.
    Kostrov, S. V.
    Sharipova, M. R.
    MICROBIOLOGY, 2006, 75 (05) : 557 - 562
12345