DNA damage and repair in individual cells: applications of the comet assay in radiobiology

被引：262
|
作者：
Olive, PL [1 ]
机构：
[1] British Columbia Canc Res Ctr, Dept Med Biophys, Vancouver, BC V5Z 1L3, Canada
来源：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1999年 / 75卷 / 04期
关键词：
D O I：
10.1080/095530099140311
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The comet assay is a single-cell gel electrophoresis method that can measure a variety of types of DNA damage, and repair of damage, in individual cells. It is now in widespread use in genetic toxicology and oncology. This review describes the history of the development of this method and its applications in radiation biology, with particular emphasis on the use of the comet assay to measure heterogeneity in DNA damage in cells exposed to ionizing radiation.
引用
收藏
页码：395 / 405
页数：11
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