Expression, purification, crystallization and preliminary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus

被引：5
|
作者：
Miyafusa, Takamitsu [1 ]
Tanaka, Yoshikazu [1 ,2 ]
Kuroda, Makoto [3 ]
Ohta, Toshiko [3 ]
Tsumoto, Kouhei [1 ]
机构：
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Tokyo 2778562, Japan
[2] Hokkaido Univ, Sapporo, Hokkaido 0010021, Japan
[3] Univ Tsukuba, Grad Sch Comprehens Human Sci, Inst Basic Med Sci, Dept Microbiol, Tsukuba, Ibaraki 3058575, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷
关键词：
Capsular polysaccharide-synthesis enzymes; Differential scanning calorimetry; Staphylococcus aureus;
D O I：
10.1107/S174430910801213X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Capsular polysaccharides (CPs) are important virulence factors of Staphylococcus aureus. The biosynthesis of type 5 and type 8 CPs (CP5 and CP8), which are produced by most clinical isolates of S. aureus, is catalyzed by 16 CP-assembling proteins. One of these proteins is the enzyme CapF, which catalyzes the synthesis of UDP-N-acetyl-L-fucosamine, a component of both CP5 and CP8. Here, the cloning, expression, purification, crystallization and diffraction analysis of CapF are reported. Optimization of the crystallization conditions by differential scanning calorimetry afforded a crystal of selenomethionine-substituted CapF that diffracted to a resolution of 2.80 angstrom. The crystal belongs to space group P3(2)21, with unit-cell parameters a = b = 119.6, c = 129.5 angstrom.
引用
收藏
页码：512 / 515
页数：4
相关论文
共 50 条
  • [21] Dynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus
    Miyafusa, Takamitsu
    Caaveiro, Jose M. M.
    Tanaka, Yoshikazu
    Tsumoto, Kouhei
    FEBS LETTERS, 2013, 587 (23) : 3824 - 3830
  • [22] Expression, crystallization, and preliminary X-ray crystallographic analysis of putative SpoVG from Staphylococcus aureus
    Hyun-Hwi Kim
    Bong-Jin Lee
    Ae-Ran Kwon
    Archives of Pharmacal Research, 2010, 33 : 1285 - 1288
  • [23] Expression, Crystallization, and Preliminary X-ray Crystallographic Analysis of Putative SpoVG from Staphylococcus aureus
    Kim, Hyun-Hwi
    Lee, Bong-Jin
    Kwon, Ae-Ran
    ARCHIVES OF PHARMACAL RESEARCH, 2010, 33 (08) : 1285 - 1288
  • [24] Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus
    Khandokar, Yogesh B.
    Roman, Noelia
    Smith, Kate M.
    Srivastava, Parul
    Forwood, Jade K.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2014, 70 : 244 - 247
  • [25] Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii
    Inagaki, E
    Sakamoto, K
    Obayashi, N
    Terada, T
    Shirouzu, M
    Bessho, Y
    Kuroishi, C
    Kuramitsu, S
    Shinkai, A
    Yokoyama, S
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 169 - 171
  • [26] Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine-6-phosphate 2-epimerase from methicillin-resistant Staphylococcus aureus
    North, Rachel A.
    Kessans, Sarah A.
    Griffin, Michael D. W.
    Watson, Andrew J. A.
    Fairbanks, Antony J.
    Dobson, Renwick C. J.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2014, 70 : 650 - 655
  • [27] Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus
    Ruggiero, Alessia
    Squeglia, Flavia
    Izzo, Viviana
    Silipo, Alba
    Vitagliano, Luigi
    Molinaro, Antonio
    Berisio, Rita
    PROTEIN AND PEPTIDE LETTERS, 2010, 17 (10): : 1296 - 1299
  • [28] The target of RNAIII-activating protein (TRAP) from Staphylococcus aureus:: purification, crystallization and preliminary X-ray analysis
    Han, YH
    Kim, YG
    Kim, DY
    Ha, SC
    Lokanath, NK
    Kim, KK
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1748 (01): : 134 - 136
  • [29] Crystallization and preliminary crystallographic analysis of dUTPase from the φ11 helper phage of Staphylococcus aureus
    Leveles, Ibolya
    Rona, Gergely
    Zagyva, Imre
    Bendes, Abris
    Harmat, Veronika
    Vertessy, Beata G.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 1411 - 1413
  • [30] Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis
    Resch, Marcus
    Roth, Heide Marie
    Kottmair, Mathias
    Sevvana, Madhumati
    Bertram, Ralph
    Titgemeyer, Fritz
    Muller, Yves A.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2009, 65 : 410 - 414
12345