A Simple RP-HPLC/UV Method for Determination of Cilostazol in Polymeric Nanoparticles Suspensions: Development and Validation

A simple and effective RP-HPLC/UV method was validated for the determination of cilostazol (CIL) in polymeric nanoparticles suspensions. Chromatographic conditions consisted of a RP C-18 column and a guard cartridge system using isocratic mobile phase of acetonitrile and water (47:53, v/v) at flow rate of 1.0 mL/min with UV detection at 254 nm. The developed method was validated by determining its performance characteristics regarding specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The method was specific, linear (r = 0.9989) in a range of 10.0 to 70.0 mu g/mL with limits of detection and quantification of 0.41 and 1.23 mu g/mL, respectively. Precision was demonstrated by a relative standard deviation lower than 0.7%. Suitable accuracy (100.81 +/- 0.97%) was achieved. The method proved to be simple, rapid, robust and adequate for quantifying CIL in polymeric nanoparticles suspensions and can be used to evaluate the drug encapsulation efficiency.