Evaluation of the EntericBio CPE assay for the detection of carbapenemase-producing organisms

被引：5
|
作者：
Vanstone, G. L. [1 ]
Wey, E. [2 ]
Mack, D. [2 ]
Smith, E. R. [2 ]
Balakrishnan, I. [2 ]
机构：
[1] HSL Analyt LLP, 1 Mabledon Pl, London WC1H 9AJ, England
[2] Royal Free London NHS Fdn Trust, Pond St, London NW3 2QG, England
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2018年 / 67卷 / 12期
关键词：
ENTEROBACTERIACEAE;
D O I：
10.1099/jmm.0.000851
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Carbapenemase-producing organisms (CPOs) represent an increasing threat in healthcare facilities and detection of these organisms in the diagnostic laboratory can be challenging. The EntericBio CPE (EBCPE) real-time PCR assay (Serosep Ltd) was evaluated for the detection of NDM, KPC, OXA-48-like, VIM, IMP and GES carbapenemase genes from a panel of 145 multidrug-resistant organisms (29 NDM, 35 OXA-48, 21 VIM, 4 OXA-23, 3 KPC, 5 NDM+OXA-48, 3 GES-5, 1 OXA-23+NDM, 1 IMI, 2 IMP-1 and 41 multidrug-resistant carbapenemase-negative isolates). The EBCPE assay performed well, with 100% sensitivity and specificity for the detection of all genotypes included in the assay. Turnaround time and laboratory workflow were improved compared to culture-based assays. Users must remain aware of the limitations of molecular assays for CPO detection to ensure implementation of the most suitable CPO diagnostic pathways.
引用
收藏
页码：1728 / 1730
页数：3
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