Evaluation and application of conducting polymer entrapment on quartz crystal microbalance in flow injection immunoassay

被引：22
|
作者：
Liu, YC [1 ]
Wang, CM
Hsiung, KP
Huang, CJ
机构：
[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan
[2] Ind Technol Res Inst, Ctr Biomed Engn, Hsinchu 300, Taiwan
[3] Natl Chung Hsing Univ, Grad Inst Vet Microbiol, Taichung 402, Taiwan
来源：
BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 07期
关键词：
quartz crystal microbalance; flow-injection-analysis; conducting polymer entrapment; pseudorabies virus; point-of-care testing;
D O I：
10.1016/S0956-5663(02)00208-7
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
An immunosensor employing conducting polymer entrapment (CPE) method to immobilize immuno-protein on the quartz crystal microbalance (QCM) for clinical flow-injection-analysis (FIA) purpose was exploited. By comparing the CPE approach and the conventional physical immobilization (PI) method, a frequency shift of the former was 47% higher than that of the latter when measuring at 0.5 mg/ml human serum albumin antibody concentration. This implied that CPE was a feasible approach for developing QCM-FIA process. An immunoassay of anti-pseudorabies virus antibody in mouse sera further exemplified its practical potential in diagnostic implication. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：937 / 942
页数：6
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