CE-MS for Proteomics and Intact Protein Analysis

被引：10
|
作者：
Kuzyk, Valeriia O. [1 ]
Somsen, Govert W. [1 ]
Haselberg, Rob [1 ]
机构：
[1] Vrije Univ Amsterdam, AIMMS Amsterdam Inst Mol & Life Sci, Div Bioanalyt Chem, Amsterdam, Netherlands
来源：
SEPARATION TECHNIQUES APPLIED TO OMICS SCIENCES: FROM PRINCIPLES TO RELEVANT APPLICATIONS | 2021年 / 1336卷
关键词：
CE-MS; Proteomics; Intact protein analysis; Sample concentration techniques; CE separation optimization; CE-MS interfacing; MS fragmentation; PTM analysis; Biopharmaceuticals analysis; Biomarker discovery; ELECTROPHORESIS-MASS-SPECTROMETRY; CAPILLARY-ZONE-ELECTROPHORESIS; ASSISTED-LASER-DESORPTION/IONIZATION; DYNAMIC PH JUNCTION; ELECTROKINETIC SUPERCHARGING PRECONCENTRATION; HIGH-SENSITIVITY CAPILLARY; TOP-DOWN CHARACTERIZATION; ANTIBODY DRUG CONJUGATE; SHEATH-FLOW INTERFACE; HUMAN-BODY FLUIDS;
D O I：
10.1007/978-3-030-77252-9_4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This chapter aims to explore various parameters involved in achieving high-end capillary electrophoresis hyphenated to mass spectrometry (CE-MS) analysis of proteins, peptides, and their posttranslational modifications. The structure of the topics discussed in this book chapter is conveniently mapped on the scheme of the CE-MS system itself, starting from sample preconcentration and injection techniques and finishing with mass analyzer considerations. After going through the technical considerations, a variety of relevant applications for this analytical approach are presented, including posttranslational modifications analysis, clinical biomarker discovery, and its growing use in the biotechnological industry.
引用
收藏
页码：51 / 86
页数：36
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