Correction accuracy in image-based adaptive optics for nonlinear microscopy

被引：0
|
作者：
Facomprez, Aurelie [1 ,2 ]
Beaurepaire, Emmanuel [1 ,2 ]
Debarre, Delphine [1 ,2 ]
机构：
[1] Ecole Polytech ParisTech, Lab Opt & Biosci, CNRS, F-91128 Palaiseau, France
[2] INSERM, F-91128 Palaiseau, France
来源：
THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XIX | 2012年 / 8227卷
关键词：
Adaptive optics; aberration correction; multiphoton microscopy; nonlinear microscopy; ABERRATION CORRECTION;
D O I：
10.1117/12.908647
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.
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收藏
页数：11
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