Vector potential of the salmon louse Lepeophtheirus salmonis in the transmission of infectious haematopoietic necrosis virus (IHNV)

被引:36
|
作者
Jakob, E. [1 ,2 ]
Barker, D. E. [2 ]
Garver, K. A. [1 ]
机构
[1] Fisheries & Oceans Canada, Pacific Biol Stn, Nanaimo, BC V9T 6N7, Canada
[2] Vancouver Isl Univ, Fisheries & Aquaculture Dept, Nanaimo, BC V9R 5S5, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Sea lice; Lepeophteirus salmonis; Infectious heamatopoietic necrosis virus (IHNV); Disease vector; Virus transmission; Atlantic salmon; Salmo salar; FARMED ATLANTIC SALMON; EPC CELL-LINE; ONCORHYNCHUS-NERKA; COPEPODA-CALIGIDAE; PISCICOLA-GEOMETRA; BRITISH-COLUMBIA; SPRING VIREMIA; SEA LICE; SALAR; CARP;
D O I
10.3354/dao02414
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
To better understand the role of vector transmission of aquatic viruses, we established an in vivo virus-parasite challenge specifically to address (1) whether Lepeophtheirus salmonis can acquire infectious haematopoietic necrosis virus (IHNV) after water bath exposure or via parasitizing infected Atlantic salmon Salmo salar and if so, define the duration of this association and (2) whether L. salmonis can transmit IHNV to naive Atlantic salmon and whether this transmission requires attachment to the host. Salmon lice which were water bath-exposed to 1 x 10(5) plaque-forming units (pfu) ml(-1) of IHNV for 1 h acquired the virus (2.1 x 10(4) pfu g(-1)) and remained IHNV-positive for 24 h post exposure. After parasitizing IHNV-infected hosts (viral titer in fish mucus 3.3 x 10(4) pfu ml(-1)) salmon lice acquired IHNV (3.4 x 10(3) pfu g(-1)) and remained virus-positive for 12 h. IHNV-positive salmon lice generated through water bath exposure or after parasitizing infected Atlantic salmon successfully transmitted IHNV, resulting in 76.5 and 86.6% of the exposed Atlantic salmon testing positive for IHNV, respectively. In a second experiment, only salmon lice that became IHNV-positive through water bath exposure transmitted IHNV to 20% of the naive fish, and no virus was transmitted when IHNV-infected salmon lice were cohabitated but restrained from attaching to naive fish. Under laboratory conditions, adult L. salmonis can acquire IHNV and transmit it to naive Atlantic salmon through parasitism. However, the ephemeral association of IHNV with L. salmonis indicates that the salmon louse act as a mechanical rather than a biological vector or reservoir.
引用
收藏
页码:155 / 165
页数:11
相关论文
共 50 条
  • [21] Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)
    Khan, Muhammad Tanveer
    Dalvin, Sussie
    Nilsen, Frank
    Male, Rune
    JOURNAL OF LIPID RESEARCH, 2017, 58 (08) : 1613 - 1623
  • [22] Development of the salmon louse Lepeophtheirus salmonis and its effects on juvenile sockeye salmon Oncorhynchus nerka
    Jakob, E.
    Sweeten, T.
    Bennett, W.
    Jones, S. R. M.
    DISEASES OF AQUATIC ORGANISMS, 2013, 106 (03) : 217 - 227
  • [23] Ionotropic receptors signal host recognition in the salmon louse (Lepeophtheirus salmonis, Copepoda)
    Komisarczuk, Anna Z.
    Grotmol, Sindre
    Nilsen, Frank
    PLOS ONE, 2017, 12 (06):
  • [24] Organisation of trypsin genes in the salmon louse (Lepeophtheirus salmonis, Crustacea, copepoda) genome
    Kvamme, BO
    Kongshaug, H
    Nilsen, F
    GENE, 2005, 352 : 63 - 74
  • [25] Candidate genes for monitoring hydrogen peroxide resistance in the salmon louse, Lepeophtheirus salmonis
    Agusti-Ridaura, Celia
    Bakke, Marit Jorgensen
    Helgesen, Kari Olli
    Sundaram, Arvind Y. M.
    Bakke, Sigrid Jorgensen
    Kaur, Kiranpreet
    Horsberg, Tor Einar
    PARASITES & VECTORS, 2020, 13 (01)
  • [26] The settlement and survival of the salmon louse, Lepeophtheirus salmonis (Kroyer, 1837), on atypical hosts
    Pert, Campbell C.
    , A. Jennifer Mordue
    Fryer, Robert J.
    O'Shea, Brid
    Bricknell, Ian R.
    AQUACULTURE, 2009, 288 (3-4) : 321 - 324
  • [27] Candidate genes for monitoring hydrogen peroxide resistance in the salmon louse, Lepeophtheirus salmonis
    Celia Agusti-Ridaura
    Marit Jørgensen Bakke
    Kari Olli Helgesen
    Arvind Y. M. Sundaram
    Sigrid Jørgensen Bakke
    Kiranpreet Kaur
    Tor Einar Horsberg
    Parasites & Vectors, 13
  • [28] Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates
    Johansson, Tove
    Einer-Jensen, Katja
    Batts, William
    Ahrens, Peter
    Bjorkblom, Carina
    Kurath, Gael
    Bjorklund, Harry
    Lorenzen, Niels
    DISEASES OF AQUATIC ORGANISMS, 2009, 86 (03) : 213 - 221
  • [29] Comparison of infectious haematopoietic necrosis virus (IHNV) isolation on monolayers and in suspended cells
    Hostnik, P
    Jencic, V
    DISEASES OF AQUATIC ORGANISMS, 2000, 40 (03) : 225 - 228
  • [30] Gene expression in five salmon louse (Lepeophtheirus salmonis, Kroyer 1837) tissues
    Edvardsen, Rolf Brudvik
    Dalvin, Sussie
    Furmanek, Tomasz
    Malde, Ketil
    Maehle, Stig
    Kvamme, Bjorn Olav
    Skern-Mauritzen, Rasmus
    MARINE GENOMICS, 2014, 18 : 39 - 44