A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish

被引：21
|
作者：
Kim, Pyeong An [1 ]
Choe, Donghwan [1 ]
So, Haeri [1 ]
Park, Soyoung [1 ]
Suh, Boeon [1 ]
Jeong, Soomin [2 ]
Kim, Ki-Tae [2 ]
Kim, Cheal [1 ]
Harrison, Roger G. [3 ]
机构：
[1] Seoul Natl Univ Sci & Technol, Dept Fine Chem, Seoul 137743, South Korea
[2] Seoul Natl Univ Sci & Technol, Dept Environm Engn, Seoul 01187, South Korea
[3] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 261卷
关键词：
Hypochlorite sensor; Fluorescent probe; Zebrafish; Imine oxidation; LIVE-CELL; PROBE; ACID; MEDIATOR; HCLO;
D O I：
10.1016/j.saa.2021.120059
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Hypochlorite is used as a water disinfectant and it is also produced by biological organisms. Its detection and quantification is important and could lead to its mechanism of reactivity in cells. We have synthesized a new fluorescence sensor for hypochlorite based on bithiophene and furan-carbohydrazide. The sensor shows increased fluorescence as a function of hypochlorite and is selective for hypochlorite. Fluorescence enhancement due to hypochlorite is observed when the sensor is used in aqueous solutions at neutral pH values. Using the sensor, the detection limit for hypochlorite is 4.2 mM, making the sensor practical to determine hypochlorite in water. Applying the sensor to aide in the detection of hypochlorite in zebrafish, showed localization of ClO-/HClO in the air bladders and eyes of zebrafish. (C) 2021 Elsevier B.V. All rights reserved.
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页数：6
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