Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library

被引：7
|
作者：
Ven Chang, Iou [1 ]
Tsutsumi, Hiroshi [2 ]
Mihara, Hisakazu [2 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Bioengn, Midori Ku, Nagatsuta Cho 4259-B40, Yokohama, Kanagawa 2268501, Japan
[2] Tokyo Inst Technol, Sch Life Sci & Technol, Midori Ku, Nagatsuta Cho 4259-B40, Yokohama, Kanagawa 2268501, Japan
来源：
MOLECULAR BIOSYSTEMS | 2017年 / 13卷 / 11期
关键词：
MONOSACCHARIDE-MODIFIED PEPTIDE; CARBOHYDRATE-BINDING PROTEINS; LIGANDS;
D O I：
10.1039/c7mb00495h
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mannose-modified lectin-binding peptides were obtained from an alpha-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies described in this study will enhance the selection of saccharide-modified ligands through the synergistic effects of sugar and peptide units, with better specificity and affinity towards lectin proteins.
引用
收藏
页码：2222 / 2225
页数：4
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