Construction of a Bacterial Artificial Chromosome (BAC) library and the genomic analysis of valosine-containing proteins in the earthworm Eisenia fetida

被引：1
|
作者：
Suzuki, Kohei [1 ,2 ]
Suzuki, Tomoharu [1 ]
Sturzenbaum, Stephen [3 ]
Gamou, Shinobu [1 ]
机构：
[1] Kyorin Univ, Sch Hlth Sci, Dept Environm & Life Sci, Tokyo 1928508, Japan
[2] STAFF Inst, Res Div 2, Tsukuba, Ibaraki 3050854, Japan
[3] Kings Coll London, Dept Biochem, Sch Biomed & Hlth Sci, London SE1 9NH, England
来源：
EUROPEAN JOURNAL OF SOIL BIOLOGY | 2008年 / 44卷 / 02期
关键词：
earthworm; BAC library; genome structure; VCP; spermatogenesis;
D O I：
10.1016/j.ejsobi.2007.12.002
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
In an attempt to isolate gonad-related genes from the earthworm Eisenia fetida (Savigny, 1826), we have isolated two genes with sequence similarities to mammalian valosine-containing proteins (evcp-1 and evcp-2) [T. Suzuki, M. Honda, S. Matsumoto, S.R. Sturzen-baum, S. Gamou. Valosine-containing proteins (VCP) in an annelid: identification of a novel spermatogenesis related factor, Gene 362 (2005) 11-18]. To understand the origin of the earthworm ucp genes, and to provide tools for further detailed genetic and physiological analyses, a Bacterial Artificial Chromosome (BAC) library was established, and the clones carrying the entire evcp-1 and evcp-2 genes were isolated. Genomic sequencing revealed that evcp-1 and evcp-2 occupied approximately 16 kb and 18 kb, respectively, and consisted of 14 introns and 15 exons, a number that is similar to mammalian VCPs. (c) 2007 Elsevier Masson SAS. All rights reserved.
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收藏
页码：202 / 206
页数：5
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