A mecA-Negative Strain of Methicillin-Resistant Staphylococcus aureus with High-Level β-Lactam Resistance Contains Mutations in Three Genes

被引：85

作者：

Banerjee, Ritu ^{[1
]}

Gretes, Michael ^{[2
]}

Harlem, Christopher ^{[3
]}

Basuino, Li ^{[3
]}

Chambers, Henry F. ^{[3
]}

机构：

[1] Mayo Clin, Div Pediat Infect Dis, Rochester, MN 55905 USA

[2] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC, Canada

[3] San Francisco Gen Hosp, Div Infect Dis, San Francisco, CA 94110 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 11期

关键词：

PENICILLIN-BINDING PROTEINS; STREPTOCOCCUS-PNEUMONIAE; VANCOMYCIN RESISTANCE; ESCHERICHIA-COLI; CEFTOBIPROLE; DOMAIN; PBP4; SUSCEPTIBILITY; EXPRESSION; ACRB;

D O I：

10.1128/AAC.00594-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of beta-lactams but was hypersusceptible to cefoxitin.

引用

页码：4900 / 4902

页数：3

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