Effect of different formulations of Rhizobium japonicum on yield and yield attributes of soybean (Glycine max L.)

One of the ways to achieve available biological nitrogen is the use of Rhizobiums for root symbiosis in legumes. Rhizobium needs a carbon source for nitrogenase enzyme protection. However, different Rhizobium japonicum formulations can be effective on N-2 fixation. Here, we applied powder and liquid formulation of Rhizobium to demonstrate that is essential for effective N-2 fixation. This is independent for root development due to their known regulation of glutamate synthesis, that is the main parameter on nitrogen fixation in soybean yield production. In our study, liquid formulation had high yield, seeds/pod and had a higher root development than other treatments. In addition, the powder formulation had a positive effect for soybean yield as compared to 250 kg urea/ha. Control plants regulated their development by low nitrogen as starter. This makes it a critical point for regulation of symbiosis.