Melatonin: quantitative analysis in pharmaceutical oral dosage forms using thin-layer chromatography (TLC) densitometry

被引：12
|
作者：
Costantini, A [1 ]
Paoli, F [1 ]
机构：
[1] Stabilimento Chim Farmaceut Militare, I-50141 Florence, Italy
来源：
FARMACO | 1998年 / 53卷 / 06期
关键词：
melatonin; densitometry; thin-layer chromatography; oral dosage forms;
D O I：
10.1016/S0014-827X(98)00040-8
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A thin-layer chromatography densitometric method for the quantitative analysis of melatonin in tablets and capsules has been developed. High-performance thin-layer chromatography (HPTLC) silica gel plates were used as the stationary phase and the elution was made with CH2Cl2/MeOH (95:5). Densitometric evaluation of the spots was performed with a TLC scanner in reflectance mode at 223 nm with a deuterium lamp. A linear relation between the peak areas and the amount of melatonin deposited was found in the range 63-399 ng. The method was applied with success for the quantitative determination of melatonin in commercial and experimental samples. (C) 1998 Elsevier Science S.A. All rights reserved.
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页码：443 / 447
页数：5
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