A new set of versatile vectors for the heterologous expression of foreign genes using the baculovirus system

被引：15
|
作者：
Lenhard, T [1 ]
Maul, G [1 ]
Haase, W [1 ]
Reilander, H [1 ]
机构：
[1] MAX PLANCK INST BIOPHYS,ABT MOLEK MEMBRANBIOL,D-60528 FRANKFURT,GERMANY
来源：
GENE | 1996年 / 169卷 / 02期
关键词：
heterologous expression; immunological detection; purification; melittin; peptide tag;
D O I：
10.1016/0378-1119(95)00831-4
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A new set of versatile advanced baculovirus (BV) vectors for the production of fused proteins in insect cells, under the control of the strong polyhedrin promoter of the Autographa california nuclear polyhedrosis virus (AcMNPV), has been constructed. The vectors contain peptide tags which allow immunological detection, as well as purification of recombinant protein produced via the BV expression system.
引用
收藏
页码：187 / 190
页数：4
相关论文
共 50 条
  • [21] Expression of human immunodeficiency virus genes using baculovirus expression system
    Yong Kang C.
    Molecular Biotechnology, 1997, 8 (2) : 173 - 187
  • [22] Baculovirus expression vectors that incorporate the foreign protein into viral occlusion bodies
    Je, YH
    Jin, BR
    Park, HW
    Roh, JY
    Chang, JH
    Seo, SJ
    Olszewski, JA
    O'Reilly, DR
    Kang, SK
    BIOTECHNIQUES, 2003, 34 (01) : 81 - +
  • [23] BACULOVIRUS VECTOR-MEDIATED EXPRESSION OF HETEROLOGOUS GENES IN INSECT CELLS
    SRIDHAR, P
    AWASTHI, AK
    AZIM, CA
    BURMA, S
    HABIB, S
    JAIN, A
    MUKHERJEE, B
    RANJAN, A
    HASNAIN, SE
    JOURNAL OF BIOSCIENCES, 1994, 19 (05) : 603 - 614
  • [24] Successful production of pseudotyped rAAV vectors using a modified baculovirus expression system
    Kohlbrenner, E
    Aslanidi, G
    Nash, K
    Shklyaev, S
    Campbell-Thompson, M
    Byrne, BJ
    Snyder, RO
    Muzyczka, N
    Warrington, KH
    Zolotukhin, S
    MOLECULAR THERAPY, 2005, 12 (06) : 1217 - 1225
  • [25] DICISTRONIC POLIOVIRUSES AS EXPRESSION VECTORS FOR FOREIGN GENES
    ALEXANDER, L
    LU, HH
    GROMEIER, M
    WIMMER, E
    AIDS RESEARCH AND HUMAN RETROVIRUSES, 1994, 10 : S57 - S60
  • [26] pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants
    Sainsbury, Frank
    Thuenemann, Eva C.
    Lomonossoff, George P.
    PLANT BIOTECHNOLOGY JOURNAL, 2009, 7 (07) : 682 - 693
  • [27] Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System
    Bruder, Mark R. R.
    Aucoin, Marc G. G.
    VIRUSES-BASEL, 2022, 14 (12):
  • [28] THE CONSTRUCTION OF A HIGHLY EFFICIENT AND VERSATILE SET OF MAMMALIAN EXPRESSION VECTORS
    STEPHENS, PE
    COCKETT, MI
    NUCLEIC ACIDS RESEARCH, 1989, 17 (17) : 7110 - 7110
  • [29] Expression of recombinant proteins in plants by using baculovirus vectors
    Eun-Yi Oh
    Young Kwan Kim
    Da-Young Park
    Zhe Lu
    Young Kug Choo
    Yeon Soo Han
    Jeong Mee Park
    Kisung Ko
    Horticulture, Environment, and Biotechnology, 2011, 52 : 95 - 104
  • [30] HETEROLOGOUS EXPRESSION OF ACTIVE MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM USING THE BACULOVIRUS EXPRESSION SYSTEM
    PEASE, EA
    AUST, SD
    TIEN, M
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (02) : 897 - 903
12345