Production of a novel bioflocculant by fed-batch culture of Citrobacter sp.

被引：76
|
作者：
Jang, JH [1 ]
Ike, M [1 ]
Kim, SM [1 ]
Fujita, M [1 ]
机构：
[1] Osaka Univ, Dept Environm Engn, Suita, Osaka 5650871, Japan
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 08期
关键词：
bioflocculant; Citrobacter; C/N ratio; dissolved O-2; fed-batch;
D O I：
10.1023/A:1010312607171
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Production of a novel bioflocculant by a fed-batch culture of Citrobacter sp. TKF04 was investigated using acetic acid as a sole carbon source. Synthesis of the bioflocculant was favored by dissolved O-2 tension at 20% of air saturation and C/N ratio (mol acetic acid/mol ammonium) of 10:1 in the feed solution. Under optimal conditions, 4.6 g crude bioflocculant per liter broth was produced, whose flocculating activity was 22 300 units. This activity was 9 times higher than that of the control (only acetic acid was supplied).
引用
收藏
页码：593 / 597
页数：5
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