High-performance liquid chromatographic determination of Roxithromycin in tablets

For the quantitative determination of Roxithromycin in tablets a rapid, and simple HPLC assay was developed. Reversed-phase chromatography was conducted using a RPC 18 (3.9 x 150 mm, 5 mu m) and guard column RP C 18 (3.0 x 3.9 mm, 5 mu m), mobile phase of 0.067 M phosphate buffer, pH 4.0 and methanol (65:35), and UV detection at 210 nm. Mean recovery of 100.90% and percentual coefficient of variation (CV%) of 1.51% was obtained from five commercial samples of Roxithromycin. The calibration graph was a straight line (r = 0.9995). Linearity is observed in the concentration range from 50.0 to 250.0 mu g/ml. The excipients did not interfere in the determination.