In this paper, indoor sand culture was conducted to study the response of different tobacco types to the mixed heavy metal tolerance. The results showed that the various of heavy metals were mainly distributed in the tobacco roots and stems after mixed heavy metal culturing. To the Burley hybrids, Cu was richest in the stem, and Pb, Cd, Cr and Zn was richest in the root; to the Burley, Cu, Pb, Cd, Cr, Zn were the highest in the stem; for the oriental tobacco, contrary to the burley, Cu, Pb, Cd, Cr, Zn were the highest in the root. Among the three tobacco types, Burley was the strongest tolerant tobacco type to Cu, Pb, Cd, Cr and Zn in the stem, especially Pb, its value up to 7061.89mg.kg(-1); After mixed metal culturing, there was no significant difference among three types of dry weight of tobacco. Therefore, the Burley was stronger one than the others in the tolerance and richness of Pb, and was considered as a plant material which could remend Pb pollution.