Cyclopropanation of Membrane Unsaturated Fatty Acids Is Not Essential to the Acid Stress Response of Lactococcus lactis subsp. cremoris

被引:41
|
作者
Thi Mai Huong To
Grandvalet, Cosette [2 ]
Tourdot-Marechal, Raphaelle [1 ]
机构
[1] Univ Bourgogne, Lab Rech Vigne & Vin EA 4149, Inst Univ Vigne & Vin Jules Guyot, IUVV, F-21078 Dijon, France
[2] AgroSup Dijon, Dept DSAN, F-21000 Dijon, France
关键词
OENOCOCCUS-OENI CELLS; ESCHERICHIA-COLI; PSEUDOMONAS-PUTIDA; SYNTHASE GENE; HEAT-SHOCK; LOW PH; LIPIDS; ADAPTATION; ETHANOL; TRANS;
D O I
10.1128/AEM.02518-10
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cyclopropane fatty acids (CFAs) are synthetized in situ by the transfer of a methylene group from S-adenosyl-L-methionine to a double bond of unsaturated fatty acid chains of membrane phospholipids. This conversion, catalyzed by the Cfa synthase enzyme, occurs in many bacteria and is recognized to play a key role in the adaptation of bacteria in response to a drastic perturbation of the environment. The role of CFAs in the acid tolerance response was investigated in the lactic acid bacterium Lactococcus lactis MG1363. A mutant of the cfa gene was constructed by allelic exchange. The cfa gene encoding the Cfa synthase was cloned and introduced into the mutant to obtain the complemented strain for homologous system studies. Data obtained by gas chromatography (GC) and GC-mass spectrometry (GC-MS) validated that the mutant could not produce CFA. The CFA levels in both the wild-type and complemented strains increased upon their entry to stationary phase, especially with acid-adapted cells or, more surprisingly, with ethanol-adapted cells. The results obtained by performing quantitative reverse transcription-PCR (qRT-PCR) experiments showed that transcription of the cfa gene was highly induced by acidity (by 10-fold with cells grown at pH 5.0) and by ethanol (by 9-fold with cells grown with 6% ethanol) in comparison with that in stationary phase. Cell viability experiments were performed after an acidic shock on the mutant strain, the wild-type strain, and the complemented strain, as a control. The higher viability level of the acid-adapted cells of the three strains after 3 h of shock proved that the cyclopropanation of unsaturated fatty acids is not essential for L. lactis subsp. cremoris survival under acidic conditions. Moreover, fluorescence anisotropy data showed that CFA itself could not maintain the membrane fluidity level, particularly with ethanol-grown cells.
引用
收藏
页码:3327 / 3334
页数:8
相关论文
共 50 条
  • [41] Lactococcus lactis subsp. lactis boosts stress resistance and host defense mechanisms in Caenorhabditis elegans
    Ali, Mohammad Shaokat
    Ahmed, Shamima
    Tanimoto, Yoshihiko
    Wada, Takayuki
    Kage-Nakadai, Eriko
    JOURNAL OF APPLIED MICROBIOLOGY, 2025, 136 (01)
  • [42] Correction to: Effects of Lactococcus lactis subsp. cremoris YRC3780 daily intake on the HPA axis response to acute psychological stress in healthy Japanese men
    Noriko Matsuura
    Hidemasa Motoshima
    Kenji Uchida
    Yujiro Yamanaka
    European Journal of Clinical Nutrition, 2022, 76 : 1492 - 1492
  • [43] Suppressive Effect of Lactococcus lactis subsp. cremoris YRC3780 on a Murine Model of Japanese Cedar Pollinosis
    Uchida, Kenji
    Iida, Kenichi
    Fujioka, Ikumi
    Hachimura, Satoshi
    Kaminuma, Osamu
    PATHOGENS, 2022, 11 (11):
  • [44] Rheological, microscopic and primary chemical characterisation of the exopolysaccharide produced by Lactococcus lactis subsp. cremoris DPC6532
    Costa, N. E.
    Wang, L.
    Auty, M. E.
    Hannon, J. A.
    McSweeney, P. L. H.
    Beresford, T. P.
    DAIRY SCIENCE & TECHNOLOGY, 2012, 92 (03) : 219 - 235
  • [45] Engineering of EPA/DHA omega-3 fatty acid production by Lactococcus lactis subsp cremoris MG1363
    Amiri-Jami, Mitra
    LaPointe, Gisele
    Griffiths, Mansel W.
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (07) : 3071 - 3080
  • [46] Influence of carbohydrate starvation and arginine on culturability and amino acid utilization of Lactococcus lactis subsp. lactis
    Stuart, MR
    Chou, LS
    Weimer, BC
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999, 65 (02) : 665 - 673
  • [47] Donkey Milk Fermentation by Lactococcus lactis subsp. cremoris and Lactobacillus rhamnosus Affects the Antiviral and Antibacterial Milk Properties
    Cirrincione, Simona
    Luganini, Anna
    Lamberti, Cristina
    Manfredi, Marcello
    Cavallarin, Laura
    Giuffrida, Maria Gabriella
    Pessione, Enrica
    MOLECULES, 2021, 26 (16):
  • [48] Prickly pear cactus as a raw material for lactic acid production by Lactococcus lactis subsp. lactis
    Tamine, Milouda
    Nancib, Aicha
    Nancib, Nabil
    Boudrant, Joseph
    MALAYSIAN JOURNAL OF MICROBIOLOGY, 2018, 14 (01) : 16 - 24
  • [49] 10-hydroxyoctadecanoic acid produced by Lactococcus lactis subsp. lactis as a part of flocculent aggregate
    Park, HJ
    Lim, YH
    Kim, YS
    Kyung, KH
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 9 (01) : 39 - 43
  • [50] Purification and characterization of x-prolyl-dipeptidyl aminopeptidase from Lactococcus lactis subsp. cremoris NRRL 634
    Alfredo E. Pérez-Guzmán
    Teresa Cruz y Victoria
    Ramón Cruz-Camarillo
    Humberto Hernández-Sánchez
    World Journal of Microbiology and Biotechnology, 2006, 22 : 953 - 958