Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers

被引：13
|
作者：
Carmon, G. [1 ,2 ]
Fishov, I. [3 ]
Feingold, M. [1 ,2 ]
机构：
[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Ilse Katz Ctr Nanotechnol, IL-84105 Beer Sheva, Israel
[3] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
来源：
OPTICS LETTERS | 2012年 / 37卷 / 03期
关键词：
ESCHERICHIA-COLI; Z RING; MICROSCOPY; DYNAMICS;
D O I：
10.1364/OL.37.000440
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments. (C) 2012 Optical Society of America
引用
收藏
页码：440 / 442
页数：3
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