Comparison of microcapillary cytometry technology and flow cytometry for CD4+ and CD8+ T-cell estimation

被引：19
|
作者：
Kandathil, AJ
Kannangai, R
David, S
Nithyanandam, G
Solomon, S
Balakrishnan, P
Abraham, OC
Subramanian, S
Rupali, P
Verghese, VP
Pulimood, S
Sridharan, G [1 ]
机构：
[1] Christian Med Coll & Hosp, Dept Clin Virol, Vellore 632004, Tamil Nadu, India
[2] Christian Med Coll & Hosp, Internal Med Unit 1, Vellore 632004, Tamil Nadu, India
[3] Christian Med Coll & Hosp, Child Hlth Unit 1, Vellore 632004, Tamil Nadu, India
[4] Christian Med Coll & Hosp, Dept Dermatol, Vellore 632004, Tamil Nadu, India
[5] VHS, YRG Care Infect Dis Lab, Madras, Tamil Nadu, India
来源：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2005年 / 12卷 / 08期
关键词：
D O I：
10.1128/CDLI.12.8.1006-1009.2005
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
An alternative technology for the estimation of T cells based on a microcapillary technique (Guava Technologies, Hayward, CA) was compared to FACSCount (Becton. Dickinson, San Jose, CA). Samples from 51 human immunodeficiency virus-infected and 21 healthy individuals were tested. The correlation (r) of the two systems for CD4(+) T cells was 0.994, and the coefficient of variation was 6.5%, establishing equable performance between the two technologies.
引用
收藏
页码：1006 / 1009
页数：4
相关论文
共 50 条
  • [41] On the role of CD4+ T cells in the CD8+ T-cell response elicited by recombinant adenovirus vaccines
    Yang, Teng Chih
    Millar, James
    Groves, Timothy
    Zhou, Wenzhong
    Grinshtein, Natalie
    Parsons, Robin
    Evelegh, Carole
    Xing, Zhou
    Wan, Yonghong
    Bramson, Jonathan
    MOLECULAR THERAPY, 2007, 15 (05) : 997 - 1006
  • [42] Clinical experience with a novel assay measuring cytomegalovirus (CMV)-specific CD4+ and CD8+ T-cell immunity by flow cytometry and intracellular cytokine staining to predict clinically significant CMV events
    Ralph Rogers
    Kapil Saharia
    Aditya Chandorkar
    Zoe F. Weiss
    Kendra Vieira
    Sophia Koo
    Dimitrios Farmakiotis
    BMC Infectious Diseases, 20
  • [43] DETERMINATION OF CD4+ AND CD8+ LYMPHOCYTES WITH THE CYTOSPHERE ASSAY - A COMPARATIVE-STUDY WITH FLOW-CYTOMETRY AND THE IMMUNOALKALINE PHOSPHATASE METHOD
    GERNOW, A
    LISSE, IM
    BOTTIGER, B
    CHRISTENSEN, L
    BRATTEGAARD, K
    CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1995, 76 (02): : 135 - 141
  • [44] CHANGES IN CD4+/CD8+ AFTER LEUKO-REDUCTION OF RED-CELLS BY FILTRATION AS DETERMINED BY FLOW-CYTOMETRY
    SLAY, RM
    STROMBERG, RR
    FRIEDMAN, LI
    BLOOD, 1993, 82 (10) : A590 - A590
  • [45] Function of CD8+, conventional CD4+, and regulatory CD4+ T cell identification in lung cancer
    Wei, Wei
    Su, Yanjun
    COMPUTERS IN BIOLOGY AND MEDICINE, 2023, 160
  • [46] Development of an immunogen containing CD4+/CD8+ T-cell epitopes for the prophylaxis of tegumentary leishmaniasis
    Isabela de Andrade Ferraz
    Ana Maria Ravena Severino Carvalho
    Rory Cristiane Fortes de Brito
    Bruno Mendes Roatt
    Vívian Tamietti Martins
    Daniela Pagliara Lage
    Luiza dos Reis Cruz
    Fernanda Alvarenga Cardoso Medeiros
    Denise Utsch Gonçalves
    Manoel Otávio da Costa Rocha
    Eduardo Antonio Ferraz Coelho
    Tiago Antônio de Oliveira Mendes
    Mariana Costa Duarte
    Daniel Menezes-Souza
    Applied Microbiology and Biotechnology, 2022, 106 : 4627 - 4641
  • [47] Allergic diseases exhibit various TREC number in CD4+ and CD8+ T-cell subpopulations
    Blinova, E. A.
    Kozhevnikov, V. S.
    Kozlov, V. A.
    IMMUNOLOGY, 2012, 137 : 466 - 466
  • [48] CD4+:CD8+ T-cell ratio changes in people with HIV receiving antiretroviral treatment
    Vivancos-Gallego, Maria J.
    Okhai, Hajra
    Perez-Elias, Maria J.
    Gomez-Ayerbe, Cristina
    Moreno-Zamora, Ana
    Casado, Jose L.
    Quereda, Carmen
    Martinez Sanz, Javier
    Sanchez-Conde, Matilde
    Serrano-Villar, Sergio
    Del Campo, Santos
    Dronda, Fernando
    Carlos Galan, Juan
    Sabin, Caroline A.
    Moreno, Santiago
    ANTIVIRAL THERAPY, 2020, 25 (02) : 91 - 100
  • [49] The dominant source of CD4+ and CD8+ T-cell activation in HIV infection is antigenic stimulation
    Stuart, JWTC
    Hazebergh, MD
    Hamann, D
    Otto, SA
    Borleffs, JCC
    Miedema, F
    Boucher, CAB
    de Boer, RJ
    JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2000, 25 (03) : 203 - 211
  • [50] The multifaceted role of CD4+ T cells in CD8+ T cell memory
    Laidlaw, Brian J.
    Craft, Joseph E.
    Kaech, Susan M.
    NATURE REVIEWS IMMUNOLOGY, 2016, 16 (02) : 102 - 111
12345