Hydrographic survey of the southeastern coastal margin of Lesvos Island (Greece) was conducted in January 2018 by R/V METEOR. The main morphological characteristic of the southern margin of Lesvos Island is a sub-parallel to the coast elongated basin, 30 km long, 5-10 km wide, and 700 m deep. It is interrupted by an oblique NW-SE narrow channel of 650 m depth, which separates the adjacent platform areas surrounding the longitudinal basin. The northern margin of the basin is abrupt with morphological slopes of more than 10 degrees, following the major WNW-ESE normal fault surface, running along the coastal zone, with an overall throw of more than 800 m. In contrast, the southern margin shows a gradual slope increase from 1 degrees to 5 degrees, where several shallow sub-bottom profiles across the basin have indicated the existence of some minor WNW-ESE normal faults and one reverse/strike-slip fault zone, with throws of a few up to 13 m. However, the NW-SE narrow channel intersecting the basin is bounded by two sub-vertical fault zones with sinistral strike-slip motion and throws of more than 150 m. The above structure was reflected in the mainshock of magnitude 6.3 (12/6/2017), corresponding to a WNW-ESE normal fault dipping to the SSW and the two major aftershocks of magnitude 5.2 and 5.0 corresponding to the two sub-parallel NW-SE sinistral strike-slip faults of the channel. This active deformation comprising WNW-ESE normal faults and NW-SE or NE-SW strike-slip faults is compatible with the area's overall tectonic frame at the eastern margin of the Aegean microplate.