Moment Analysis of Chromatographic Behavior of Superficially Porous Particles

被引：0

作者：

Miyabe, Kanji ^{[1
]}

Noiri, Kazuhiro ^{[2
]}

Kobayashi, Kouki ^{[2
]}

机构：

[1] Toyama Univ, Grad Sch Sci & Engn Res, Toyama 9308555, Japan

[2] Toyama Univ, Fac Engn, Toyama 9308555, Japan

来源：

ANALYTICAL SCIENCES | 2011年 / 27卷 / 11期

关键词：

MASS-TRANSFER KINETICS; DIFFUSION-COEFFICIENTS; LIQUID-CHROMATOGRAPHY; LONGITUDINAL DIFFUSION; HOMOLOGOUS SERIES; SILICA PARTICLES; STATIONARY-PHASE; SHELL PARTICLES; PEAK-CAPACITY; STOP-FLOW;

D O I：

10.2116/analsci.27.1097

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C-18-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C-18-particles were comparable to those of columns packed with a C-18-silica monolith and full-porous C-18-silica gel particles. The flow-rate dependence of HETP was hypothetically calculated by using moment equations to clarify the influence of the structural characteristics on the chromatographic behavior. The column efficiency of a column packed with the superficially porous particles is higher in the high flow-rate range than that with full-porous spherical particles. This is attributed to the smaller contribution of the intraparticulate mass transfer in the superficially porous particles to band broadening. The moment equations are effective for the quantitative analysis of chromatographic behavior of superficially porous particles.

引用

页码：1097 / 1105

页数：9

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