SINGLE-MOLECULE DUAL-BEAM OPTICAL TRAP ANALYSIS OF PROTEIN STRUCTURE AND FUNCTION

被引：23

作者：

Sung, Jongmin ^{[1
,2
]}

Sivaramakrishnan, Sivaraj ^{[1
]}

Dunn, Alexander R. ^{[3
]}

Spudich, James A. ^{[1
]}

机构：

[1] Stanford Univ, Dept Biochem, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

来源：

METHODS IN ENZYMOLOGY, VOL 475: SINGLE MOLECULE TOOLS, PT B: SUPER-RESOLUTION, PARTICLE TRACKING, MULTIPARAMETER, AND FORCE BASED METHODS | 2010年 / 475卷

关键词：

MYOSIN-V; SLIDING MOVEMENT; ACTIN-FILAMENTS; CARDIAC MYOSIN; SMOOTH-MUSCLE; POWER STROKE; FORCE; RESOLUTION; MOTOR; CONSTRUCTION;

D O I：

10.1016/S0076-6879(10)75014-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.

引用

页码：321 / 375

页数：55

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