Comparative Evaluation of the Parameters of Sperm Apoptosis of Young and Middle-Aged Men by Flow Cytometry

被引：0
|
作者：
Dolgikh, O., V [1 ]
Dianova, D. G. [1 ]
Krivtsov, A., V [1 ]
Alikina, I. N. [1 ]
机构：
[1] State Sci Ctr Med & Prevent Hlth Risk Management, Perm, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2022年 / 172卷 / 04期
关键词：
flow cytometry; spermatozoon; caspase; p53; receptor-mediated apoptosis;
D O I：
10.1007/s10517-022-05418-1
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
A comparative analysis of the parameters characterizing sperm apoptosis of young (27-42 years) and middle-aged (44-51 years) men was performed by flow cytometry. Irrespective of age, activity of caspase-3 and p53-mediated controlling the transmission of apoptogenic signal transmission in gametes remained stable with the formation of germ cells with delayed (p<0.05) cell death according to the Annexin V-FITC+PI- criterion (predominantly in middle-aged men). Inhibition of the transmission of a proapoptogenic stimulus mediated by membrane cell death receptors (FAS) was also observed in this group. Comparison of indicators of sperm apoptosis showed age-related features of cell death, in particular, inhibition of membrane reception triggering FAS-dependent apoptosis, which is associated with insufficient phosphatidylserine production in middle-aged men, excessive life cycle duration, and aging of spermatozoa.
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页码：483 / 485
页数：3
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