Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

被引:154
|
作者
Coscia, F. [1 ]
Watters, K. M. [2 ]
Curtis, M. [2 ]
Eckert, M. A. [2 ]
Chiang, C. Y. [2 ]
Tyanova, S. [1 ]
Montag, A. [3 ]
Lastra, R. R. [3 ]
Lengyel, E. [2 ]
Mann, M. [1 ]
机构
[1] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
[2] Univ Chicago, Gynecol Oncol Sect, Dept Obstet & Gynecol, Chicago, IL 60637 USA
[3] Univ Chicago Med, Dept Pathol, Chicago, IL 60637 USA
关键词
FALLOPIAN-TUBE; RETINOIC ACID; MOLECULAR MARKER; EXPRESSION; IDENTIFICATION; GROWTH; QUANTIFICATION; METASTASIS; CRYSTALLIN; CARCINOMA;
D O I
10.1038/ncomms12645
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumour of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here, we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumours, immortalized ovarian surface epithelial cells and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantification of 410,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II) and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic data set, as well as a confirmatory publicly available CPTAC/TCGA tumour proteome data set, into a predominantly epithelial and mesenchymal HGSOC tumour cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumours indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.
引用
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页数:14
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