Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies

There is no consensus as to how a precursor lesion, germ cell neoplasia in situ (GCNIS), develops into the histologic types of testicular germ cell tumor type II (TGCT). The present meta-analysis examined RNA expressions of 24 candidate genes in three datasets. They included 203 samples of normal testis (NT) and histologic types of TGCT. The Fisher's test for combinedpvalues was used for meta-analysis of the RNA expressions in the three datasets. The histologic types differed in RNA expression ofPRAME, KIT, SOX17, NANOG, KLF4, POU5F1, RB1, DNMT3B, andLIN28A(p< 0.01). The histologic types had concordant differences in RNA expression of the genes in the three datasets. Eight genes had overlap with a high RNA expression in at least two histologic types. In contrast, only seminoma (SE) had a high RNA expression ofKLF4and only embryonal carcinoma (EC) had a high RNA expression ofDNMT3B. In conclusion, the meta-analysis showed that the development of the histologic types of TGCT was driven by changes in RNA expression of candidate genes. According to the RNA expressions of the ten genes, TGCT develops from NT over GCNIS, SE, EC, to the differentiated types of TGCT.