Effects of aspirin on the in vitro and in vivo acetylation of 2-aminofluorene in Sprague-Dawley rats

被引:0
|
作者
Lin, SS
Yu, CS
Wu, JY
Tyan, YS
Hsia, TC
Lin, WC
Chung, JG [1 ]
机构
[1] China Med Univ, Sch Med, Dept Microbiol, Taichung 404, Taiwan
[2] Chungtai Inst Hlth Sci & Technol, Dept Radiol Technol, Taichung, Taiwan
[3] China Med Univ, Ctr Gen Educ, Taichung 404, Taiwan
[4] China Med Univ, Dept Pharmacol, Taichung 404, Taiwan
[5] China Med Univ, Dept Microbiol, Taichung 404, Taiwan
[6] Armed Forces Taichung Gen Hosp, Dept Chest Surg, Taichung, Taiwan
[7] Chung Shan Med Univ, Dept Radiol, Taichung, Taiwan
[8] China Med Univ Hosp, Dept Internal Med, Taichung, Taiwan
来源
IN VIVO | 2005年 / 19卷 / 02期
关键词
acetylsalicylic acid; 2-aminofluorene; N-acetyl-2-aminofluorene; N-acetyltransferase; p-aminobenzoic acid; N-acetyl-p-aminobenzoic acid;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The present study was undertaken to determine the effect of acetylsalicylic acid acid on the in vitro N-acetyltransferase (NAT) enzyme activity and in vivo acetylation of 2-aminofluorene in laboratory rats. In the in vitro experiments, cytosols of blood, bladder, colon and liver cells, with or without acetylsalicylic acid co-treatment, showed different percentages of 2-aminofluorene acetylation. The data indicated that there was decreased NAT activity associated with increased acetylsalicylic acid in the cytosol reaction. In the in vitro experiments, values of apparent Kin and Vmax decreased by 4% and 21%, respectively, for acetylation of AF in blood NAT, 28% and 31% for acetylation of AF in bladder NAT, 12% and 25% for acetylation of AF in colon NAT, and 50% and 35% for acetylation of AF in liver NAT In the in vivo experiments, pretreatment with acetylsalicylic acid (50 mg/kg) 48 h prior to the administration of 2-aminofluorene (50 mg/kg) resulted in 24% and 28% decreases in the fecal and urinary recovery of N-acetyl-2-aminofluorene and a 26% decrease in the metabolic clearance of 2-aminofluorene to N-acetyl-2-aminofluorene. This is the first demonstration of acetylsalicylic acid (Aspirin) inhibition of arylamine N-acetyltransferase activity showing decreases in the N-acetylation of carcinogens in vivo.
引用
收藏
页码:475 / 481
页数:7
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