Optical fluorescence and Raman biopsy of squamous cell carcinoma from the head and neck

Fluorescence, excitation, diffuse reflectance and FT-IR Raman spectral scans of squamous cell carcinoma of the head and neck have been studed. Eleven specimens of squamous cell carcinoma in larynx, lymph node, throat, grand, soft palate and epiglotts and nine corresponding normal specimens was tested. The excitation spectral differences between squamous cell carcinoma and normal were investigated in the protein spectral range 260 nm to 300 nm. The ratios intensity at various excitation wavelengths were used as criterion to separate carcinoma and normal tissues. The t-test of these parameter have been made. The P values of all these three parameters sue Less than 0.005. The statistical results of sensitivity and specificity of using these three parameters compared to pathological results are about 90%. The Raman spectroscopy was measured. Characteristic vibrational modes were used to separate out carcinoma and correlated with excitation spectroscopy data.