Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

被引：3
|
作者：
Ernst, D [1 ]
Liegl, I [1 ]
Kiefer, E [1 ]
Seidlitz, HK [1 ]
Heller, W [1 ]
Sandermann, H [1 ]
机构：
[1] GSF, Natl Res Ctr Environm & Hlth, Inst Biochem Plant Pathol, D-85764 Neuherberg, Germany
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 2001年 / 23卷 / 03期
关键词：
European beech; mRNA; ribosomal protein; UV-B;
D O I：
10.1007/s11738-001-0042-9
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Changes in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 290-320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypepetide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.
引用
收藏
页码：343 / 349
页数：7
相关论文
共 50 条
  • [31] Effects of drought on photoprotective mechanisms in European beech (Fagus sylvatica L.) seedlings from different provenances
    J.I. García-Plazaola
    J.M. Becerril
    Trees, 2000, 14 : 485 - 490
  • [32] Differentiation in phenological and physiological traits in European beech (Fagus sylvatica L.)
    Dušan Gömöry
    Ľubica Ditmarová
    Matúš Hrivnák
    Gabriela Jamnická
    Jaroslav Kmeť
    Diana Krajmerová
    Daniel Kurjak
    European Journal of Forest Research, 2015, 134 : 1075 - 1085
  • [33] Frost resistance and phenology of European beech provenances (Fagus sylvatica L.)
    Visnjic, C
    Dohrenbusch, A
    ALLGEMEINE FORST UND JAGDZEITUNG, 2004, 175 (06): : 101 - 108
  • [34] Variation of cytosine methylation patterns in European beech (Fagus sylvatica L.)
    Hrivnak, Matus
    Krajmerova, Diana
    Frydl, Josef
    Gomory, Dusan
    TREE GENETICS & GENOMES, 2017, 13 (06)
  • [35] Potential risks for European beech (Fagus sylvatica L.) in a changing climate
    Gessler, Arthur
    Keitel, Claudia
    Kreuzwieser, Juergen
    Matyssek, Rainer
    Seiler, Wolfgang
    Rennenberg, Heinz
    TREES-STRUCTURE AND FUNCTION, 2007, 21 (01): : 1 - 11
  • [36] Preformed and neoformed syllepsis and prolepsis of European Beech - Fagus sylvatica L.
    Gruber, F
    FLORA, 1998, 193 (04) : 369 - 385
  • [37] Potential risks for European beech (Fagus sylvatica L.) in a changing climate
    Arthur Geßler
    Claudia Keitel
    Jürgen Kreuzwieser
    Rainer Matyssek
    Wolfgang Seiler
    Heinz Rennenberg
    Trees, 2007, 21 : 1 - 11
  • [38] Differentiation in phenological and physiological traits in European beech (Fagus sylvatica L.)
    Goemoery, Dusan
    Ditmarova, L'ubica
    Hrivnak, Matus
    Jamnicka, Gabriela
    Kmet', Jaroslav
    Krajmerova, Diana
    Kurjak, Daniel
    EUROPEAN JOURNAL OF FOREST RESEARCH, 2015, 134 (06) : 1075 - 1085
  • [39] GENETIC DIFFERENTIATION OF EUROPEAN BEECH (Fagus sylvatica L.) IN BOSNIA AND HERZEGOVINA
    Ballian, Dalibor
    Bogunic, Faruk
    Mujezinovic, Osman
    Kajba, Davorin
    SUMARSKI LIST, 2012, 136 (11-12): : 587 - 595
  • [40] Does climate drive the defoliation of European beech ( Fagus sylvatica L.)?
    Rukh, Shah
    Krueger, Inken
    Potocic, Nenad
    Timmermann, Volkmar
    Bolte, Andreas
    FOREST ECOLOGY AND MANAGEMENT, 2024, 572
12345