Keth-seq for transcriptome-wide RNA structure mapping

被引：83

作者：

Weng, Xiaocheng ^{[1
,2
]}

Gong, Jing ^{[3
]}

Chen, Yi ^{[2
]}

Wu, Tong ^{[1
]}

Wang, Fang ^{[1
,4
]}

Yang, Shixi ^{[2
]}

Yuan, Yushu ^{[2
]}

Luo, Guanzheng ^{[1
]}

Chen, Kai ^{[1
]}

Hu, Lulu ^{[1
]}

Ma, Honghui ^{[1
]}

Wang, Pingluan ^{[1
]}

Zhang, Qiangfeng Cliff ^{[3
]}

Zhou, Xiang ^{[2
]}

He, Chuan ^{[1
]}

机构：

[1] Univ Chicago, Howard Hughes Med Inst, Inst Biophys Dynam, Dept Chem,Dept Biochem & Mol Biol, 5841 S Maryland Ave, Chicago, IL 60637 USA

[2] Wuhan Univ, Minist Educ, Key Lab Biomed Polymers, Coll Chem & Mol Sci, Wuhan, Peoples R China

[3] Tsinghua Univ, Ctr Synthet & Syst Biol, Tsinghua Peking Joint Ctr Life Sci,Sch Life Sci, MOE Key Lab Bioinformat,Beijing Adv Innovat Ctr S, Beijing, Peoples R China

[4] Wuhan Univ, Sch Pharmaceut Sci, Wuhan, Peoples R China

来源：

NATURE CHEMICAL BIOLOGY | 2020年 / 16卷 / 05期

基金：

美国国家卫生研究院; 中国国家自然科学基金;

关键词：

SELECTIVE 2'-HYDROXYL ACYLATION; SECONDARY STRUCTURE; REVEALS;

D O I：

10.1038/s41589-019-0459-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA secondary structure is critical to RNA regulation and function. We report a new N-3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N-3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.

引用

页码：489 / +

页数：9

共 50 条

[41] Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq
Yusheng Liu
Yiwei Zhang
Jiaqiang Wang
Falong Lu
Nature Protocols, 2022, 17 : 1980 - 2007
[42] FragSeq: transcriptome-wide RNA structure probing using high-throughput sequencing
Jason G Underwood
Andrew V Uzilov
Sol Katzman
Courtney S Onodera
Jacob E Mainzer
David H Mathews
Todd M Lowe
Sofie R Salama
David Haussler
Nature Methods, 2010, 7 : 995 - 1001
[43] FragSeq: transcriptome-wide RNA structure probing using high-throughput sequencing
Underwood, Jason G.
Uzilov, Andrew V.
Katzman, Sol
Onodera, Courtney S.
Mainzer, Jacob E.
Mathews, David H.
Lowe, Todd M.
Salama, Sofie R.
Haussler, David
NATURE METHODS, 2010, 7 (12) : 995 - U81
[44] New tools for transcriptome-wide mapping of m6A
Clyde, Dorothy
NATURE REVIEWS GENETICS, 2020, 21 (07) : 387 - 387
[45] Probabilistic fine-mapping of transcriptome-wide association studies
Nicholas Mancuso
Malika K. Freund
Ruth Johnson
Huwenbo Shi
Gleb Kichaev
Alexander Gusev
Bogdan Pasaniuc
Nature Genetics, 2019, 51 : 675 - 682
[46] New tools for transcriptome-wide mapping of m6A
Dorothy Clyde
Nature Reviews Genetics, 2020, 21 : 387 - 387
[47] Probabilistic fine-mapping of transcriptome-wide association studies
Mancuso, Nicholas
Freund, Malika K.
Johnson, Ruth
Shi, Huwenbo
Kichaev, Gleb
Gusev, Alexander
Pasaniuc, Bogdan
NATURE GENETICS, 2019, 51 (04) : 675 - +
[48] CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex
Sauliere, Jerome
Murigneux, Valentine
Wang, Zhen
Marquenet, Emelie
Barbosa, Isabelle
Le Tonqueze, Olivier
Audic, Yann
Paillard, Luc
Roest Crollius, Hugues
Le Hir, Herve
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2012, 19 (11) : 1124 - +
[49] Enhanced transcriptome-wide RNA G-quadruplex sequencing for low RNA input samples with rG4-seq 2.0
Zhao, Jieyu
Chow, Eugene Yui-Ching
Yeung, Pui Yan
Zhang, Qiangfeng Cliff
Chan, Ting-Fung
Kwok, Chun Kit
BMC BIOLOGY, 2022, 20 (01)
[50] Enhanced transcriptome-wide RNA G-quadruplex sequencing for low RNA input samples with rG4-seq 2.0
Jieyu Zhao
Eugene Yui-Ching Chow
Pui Yan Yeung
Qiangfeng Cliff Zhang
Ting-Fung Chan
Chun Kit Kwok
BMC Biology, 20

← 1 2 3 4 5 →