Systematic analysis of oxidative degradation of polysaccharides using PAGE and HPLC-MS

被引：11
|
作者：
Ovalle, R [1 ]
Soll, CE [1 ]
Lim, F [1 ]
Flanagan, C [1 ]
Rotunda, T [1 ]
Lipke, PN [1 ]
机构：
[1] CUNY Hunter Coll, Ctr Study Gene Struct & Funct, Dept Biol, New York, NY 10021 USA
来源：
CARBOHYDRATE RESEARCH | 2001年 / 330卷 / 01期
关键词：
Fenton; oxidation; HPLC; MS; FC-PAGE; carbohydrate; fluorescence; ANTS; tyrosine;
D O I：
10.1016/S0008-6215(00)00262-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxidation of polysaccharides yields hydroxyaldehydes and hydroxycarboxylic acids. Aldehydes and carboxylic acids were separately conjugated to 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) or tyrosine t-butyl ester (TBT). The ANTS-labeled derivatives were separated by molecular size on PAGE gels and detected by fluorescence. TBT-labeled derivatives were separated by reverse phase chromatography on a C-18-HPLC column and analyzed by positive ion electrospray mass spectroscopy (HPLC-MS). This combination of procedures allowed a systematic analysis of carbohydrate oxidation products. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：131 / 139
页数：9
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