A novel DNA hairpin substrate for bleomycin

被引：16
|
作者：
Akiyama, Yoshitsugu [1 ]
Ma, Qian [1 ]
Edgar, Erin [2 ]
Laikhter, Andrei [2 ]
Hecht, Sidney M. [1 ]
机构：
[1] Univ Virginia, Dept Biol & Chem, Charlottesville, VA 22904 USA
[2] Integrated DNA Technologies, Coralville, IA 52241 USA
来源：
ORGANIC LETTERS | 2008年 / 10卷 / 11期
关键词：
D O I：
10.1021/ol800445x
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A 16-nucleotide DNA hairpin containing 4-aminobenzo[g]quinazoline-2-one 2'-deoxyribose at position 15 has been prepared and found to lack significant fluorescence, When treated with Fe(II).BLM, the hairpin was found to undergo oxidative transformation selectively at position 15. The predominant fluorescent product was characterized and quantified. The pro-fluorescent DNA hairpin was used as a substrate for 15 bleomycin congeners, and the results were compared with those obtained following cleavage of a radiolabeled DNA duplex and PAGE analysis.
引用
收藏
页码：2127 / 2130
页数：4
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