Prevalence of Coxiella burnetii infection in dairy cattle with reproductive disorders

被引：98

作者：

To, H ^{[1
]}

Htwe, KK ^{[1
]}

Kako, N ^{[1
]}

Kim, HJ ^{[1
]}

Yamaguchi, T ^{[1
]}

Fukushi, H ^{[1
]}

Hirai, K ^{[1
]}

机构：

[1] Gifu Univ, Dept Vet Microbiol, Fac Agr, Gifu 5011193, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1998年 / 60卷 / 07期

关键词：

bovine coxiellosis; Coxiella burnetii; nested polymerase chain reaction;

D O I：

10.1292/jvms.60.859

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The prevalence of Coxiella burnetii infection in 207 cattle with reproductive disorders was studied by using an indirect immunofluorescence (IF) test, nested polymerase chain reaction (PCR) and isolation. IF antibodies to phase I and phase II antigens of C. burnetii were found in 122 (58.9%) and 125 (60.4%) of the sera, respectively, and PCR-positives were found in 8 (3.9%) of the sera and in 51 (24.6%) of the milk samples. In addition, C, burnetii was isolated from 51 (24.6%) of the milk samples by inoculating laboratory mice. The results indicate that the IF test plus PCR are useful in the diagnosis of bovine coxiellosis. It is difficult to deny that dairy cattle with reproductive disorders would be one of the important reservoirs of C. burnetii responsible for infection in both animal and human populations in Japan.

引用

页码：859 / 861

页数：3

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