Detection of resistance to rifampicin and decreased susceptibility to penicillin in Neisseria meningitidis by real-time multiplex polymerase chain reaction assay

被引：4
|
作者：
Stefanelli, Paola [1 ]
Neri, Arianna [1 ]
Carattoli, Alessandra [1 ]
Mastrantonio, Paola [1 ]
机构：
[1] Ist Super Sanita, Parasit & Immune Mediated Dis, Dept Infect, I-00161 Rome, Italy
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 58卷 / 02期
关键词：
Neisseria meningitidis; rifampicin; penicillin; real-time PCR;
D O I：
10.1016/j.diagmicrobio.2006.11.022
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, we designed primers and probes for the rpoB gene of Neisseria meningitidis to detect rifampicin-resistant strains in a combined use with primers and probes previously described for penicillin intermediate isolates. The assay was set up in the Light Cycler instrument using the Fluorescence Resonance Energy Transfer platform. The method was applied to N. meningitidis strains and to culture-negative cerebrospinal fluids (CSFs) from patients with meningococcal invasive disease. A spiked CSF was used to determine the sensitivity of the assay. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：241 / 244
页数：4
相关论文
共 50 条
  • [41] Evaluating the generalizability of a multiplex real-time PCR assay for predicting decreased susceptibility to ceftriaxone in a global set of Neisseria gonorrhoeae sequences
    Lin, Eric Y.
    Adamson, Paul C.
    Klausner, Jeffrey D.
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2021, 76 (04) : 1104 - 1106
  • [42] Detection and serogroup determination of Neisseria meningitidis in CSF by polymerase chain reaction (PCR)
    Porritt, RJ
    Mercer, JL
    Munro, R
    PATHOLOGY, 2000, 32 (01) : 42 - 45
  • [43] Real-time polymerase chain reaction
    Oldach, D
    GASTROENTEROLOGY, 1999, 116 (03) : 763 - 765
  • [44] Detection and differentiation of bovine herpesvirus 1 and 5 using a multiplex real-time polymerase chain reaction
    Diallo, Ibrahim S.
    Corney, Bruce G.
    Rodwell, Barry J.
    JOURNAL OF VIROLOGICAL METHODS, 2011, 175 (01) : 46 - 52
  • [45] Real-Time Polymerase Chain Reaction Assay for the Diagnosis of Pulmonary Tuberculosis
    Wiwanitkit, Viroj
    TUBERCULOSIS AND RESPIRATORY DISEASES, 2015, 78 (04) : 473 - 473
  • [46] Real-time polymerase chain reaction
    Wilhelm, J
    Pingoud, A
    CHEMBIOCHEM, 2003, 4 (11) : 1120 - 1128
  • [47] Detection of peanut using real-time polymerase chain reaction
    H. Hird
    J. Lloyd
    R. Goodier
    J. Brown
    P. Reece
    European Food Research and Technology, 2005, 220 : 238 - 238
  • [48] Detection of the FCGR3a polymorphism using a real-time polymerase chain reaction assay
    Burchard, Paul R.
    Malhotra, Saurabh
    Kaur, Prabhjot
    Tsongalis, Gregory J.
    CANCER GENETICS, 2013, 206 (04) : 130 - 134
  • [49] Detection of the pinewood nematode, Bursaphelenchus xylophilus, using a real-time polymerase chain reaction assay
    Cao, AX
    Liu, XZ
    Zhu, SF
    Lu, BS
    PHYTOPATHOLOGY, 2005, 95 (05) : 566 - 571
  • [50] Development of a real-time polymerase chain reaction assay for detection of Actinobacillus suis in porcine lung
    Kariyawasam, Subhashinie
    Strait, Erin
    Jordan, Dianna
    Kroll, Jeremy
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2011, 23 (05) : 885 - 889
12345