Purification and characterisation of galactose-induced pectinases from the exo-1 mutant strain of Neurospora crassa

被引：5
|
作者：
Crotti, LB
Jorge, JA
Terenzi, HF
Polizeli, MLTM
机构：
[1] Departmento de Biologia-Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901 Ribeirao Preto, Sao Paulo
来源：
PECTINS AND PECTINASES | 1996年 / 14卷
关键词：
D O I：
10.1016/S0921-0423(96)80317-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Pectinases produced by the exo-1 mutant of N. crassa in galactose plus glucose supplemented medium, were separated by ion-exchange chromatography into two pools. Pool I contained pectate and pectin lyases, and variable polygalacturonase activity. Pool 2 contained polygalacturonase activity only. Gel filtration indicated a MWapp of 80 kDa (higher than those of separate enzymes) for all activities in the first pool, suggesting a complex. Polygalacturonase, pectin and pectate lyases were purified 39-fold, 22-fold and 33-fold, respectively. Optima of temperature and pH were 45 degrees C and 5.5 for polygalacturonase activity and 50 degrees C and 9.5 for lyase activities. Km and Vmax values for polygalacturonase were 0.023 mg polypectate/ml and 2.08 mu moles (reducing sugar)/min/mg protein.
引用
收藏
页码：787 / 792
页数：6
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