Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

被引：3

作者：

Mun, Dasom ^{[1
]}

Kang, Ji-Young ^{[1
]}

Chun, Yumin ^{[1
]}

Park, Da-Seul ^{[1
]}

Kim, Hyoeun ^{[2
]}

Yun, Nuri ^{[3
]}

Lee, Seung-Hyun ^{[2
]}

Joung, Boyoung ^{[1
]}

机构：

[1] Yonsei Univ, Coll Med, Div Cardiol, 50-1 Yonsei Ro, Seoul 03722, South Korea

[2] Yonsei Univ, Coll Med, Dept Biochem & Mol Biol, 50-1 Yonsei Ro, Seoul 03722, South Korea

[3] Yonsei Univ, Inst Life Sci & Biotechnol, 50 Yonsei Ro, Seoul 03722, South Korea

来源：

STEM CELL RESEARCH | 2022年 / 65卷

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1016/j.scr.2022.102940

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and sponta-neous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.

引用

页数：4

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